The Process Optimization of Furostanol Saponins Extraction from the Aerial Part of Tribulus Terrestris
Keywords:
Tribulus terrestris L., furostanol saponins, extractionAbstract
Data on the research of the selection of parameters of influencing factors to the stage extraction of furostanol saponins from Tribulus terrestris in the literature are not enough. This article presents the results of research on the stage of extraction of furostanol saponins from the aerial part of Tribulis terrestris. In this study, the optimal conditions for the extraction process were used technological methods, such as studying the effect of alcohol concentration, the degree of comminuting of raw materials, temperature, and process dynamics on the yield of furostanol saponins from the aerial part of Tribulus terrestris. To optimize the extraction process, the method of mathematical modeling of the experiment according to Box–Wilson. Determination of the yield of furostanol saponins was carried out by spectrophotometry method. During the study of the process of furostanol saponins extraction from the aerial part of Tribulus terrestris, it was found that the comminuted raw materials with a particle size of 2-6 mm are necessary to extract seven times with 70% ethanol at room temperature (20-25°C), the seventh overflow of which is used to enrich subsequent extraction. The necessary time insisting at the first contact of the phases should be at least 8 hours, by the second phase contact is achieved after 7 hours, the third and fourth - 6 hours, the fifth and sixth - 4 hours, and the seventh - 2 hours. The proposed conditions of furostanol saponins extraction from the aerial part of Tribulus terrestris approved by processing 40 tons of plant raw material at the Scientific Center for Technology to International GMP Requirements at the Institute of Chemistry of Plant Substances. On the industrial scale, the introduced extraction conditions increased the extraction of furostanol saponins up to 96% of the content in the raw material.
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