Effect of Chemical Mutagens on Alpha Amylase Activity of Bacillus Amyloliquefaciens

Authors

  • U.U. Zango Department of Biology, School of Science Education, Sa’adatu Rimi College of Education, Kumbotso, P.M.B. 3218 Kano State, Nigeria
  • M. Ibrahim Department of Science Laboratory Technology, Federal Polytechnic Offa, Kwara State, Nigeria
  • M. Suleiman Department of Biological Sciences, Federal College of Education Technical, Bichi, Kano State, Nigeria

Keywords:

Amylase, acrylamide, bacillus, mutant

Abstract

In this research, the influence of different mutational treatments (Ethidium bromide (EtBr) and acrylamide treatment) on amylase production was studied. EtBr mutant showed increased in alpha amylase activity at 200µg/ml with enzyme activity compared with the wild strain at different incubation time. Increase in EtBr concentration from 200 µg/ml, 300 µg/ml and 400 µg/ml shows increase in enzyme activity 0.603455 U/ml, 0.652753 U/ml and 0.711182 U/ml respectively. Decreased in enzyme activity was observed in EtBr mutant exposed to 500 µg/ml and 600 µg/ml with enzyme activity 0.661883 U/ml and 0.579718 U/ml respectively. Acrylamide mutant showed no increase in enzyme activity at concentration of 200 µg/ml compared with wild strain at different incubation time. Increase in acrylamide concentration from 200 µg/ml, 300 µg/ml and 400 µg/ml shows increase in enzyme activity (0.267492 U/ml, 0.740396 U/ml and 0.818909 U/ml respectively). At concentrations of 500 and 600 the enzyme activity decreased with enzyme activity 0.683793 U/ml and 0.42817 U/ml.

 

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Published

2021-02-28

How to Cite

Zango, U., Ibrahim, M., & Suleiman, M. (2021). Effect of Chemical Mutagens on Alpha Amylase Activity of Bacillus Amyloliquefaciens. International Journal of Scientific Research in Chemical Sciences, 8(1), 9–16. Retrieved from https://ijsrcs.isroset.org/index.php/j/article/view/89

Issue

Vol. 8 No. 1 (2021): February

Section

Research Article

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