Inorganic Analysis and Antioxidant Activity of Shilajit`
Keywords:
shilajit composition, minerals, trace metals, phosphate content, antioxidant activityAbstract
Shilajit is an ancient Hindu medication of Ayurveda which has many applications worldwide. This study aims to investigate the inorganic content of two common samples from Pakistan and Afghanistan used in the treatment of many diseases in Kingdom of Bahrain. The inorganic phosphate content of Afghani and Pakistani shilajit samples was determined to be 0.133 ?0.004 mg/g and 0.174 ?0.002 mg/g, respectively. Elemental analysis of solid Afghani and Pakistani shilajit samples was determined by energy dispersive x-ray fluorescence (ED-XRF). Afghani shilajit sample mainly comprised of calcium (50.298%), sulfur (21.299%), potassium (17.194%) and chlorine (8.405%) while the Pakistani shilajit mainly comprised of potassium (24.309%), calcium (20.933%), chlorine (18.614%), and silicon (15.197%) with relatively close percentages. Determination of the concentration of minerals and trace metals; As, Pb, Cd, Ag, Al, B, Ba, Cr, Cu, Fe, Mn, Ni, Sb, Se, Zn, P, Ca, K, Mg and Na, in each sample was carried out by inductively coupled plasma-optical emission spectrometry (ICP-OES). According to the ICP-OES analysis, the most significant outcome was the concentration of Ca and K in Afghani shilajit, which were determined to be 30292 and 21587 ppm (equivalent to 30.292 and 21.587 g/L), respectively. The antioxidant activity of both shiljait samples were evaluated by determining 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity. The antioxidant activity of Afghani and Pakistani shilajit samples were determined to be 96.797 ? 0.561% and 95.297 ? 2.884%, respectively which may indicate strong antioxidant actions.
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