Analysis of In-vitro antioxidant properties of Green synthesized Ni/reduced graphene oxide composite
Keywords:
Nanoparticles, Azadirachta indica, Antioxidant, Reduced Graphene oxide, Phytochemicals, NickelAbstract
Nickel nanoparticles (NiNPs) possess numerous health benefits that are useful in biomedical applications. Synthesis of NPs via green approaches has become prominent nowadays due to their cost effective, and eco-friendly manner. In the current study, Ni NPs were synthesized via a green approach. As a support to disperse and stabilize NPs, reduced graphene oxide (rGO) was used while preventing agglomeration. Then the in-vitro antioxidant activity of synthesized composites was examined. As the reducing and capping agent Azadirachta indica (Neem) extract was used in the synthesis process. All Ni/rGO composites synthesized possessed antioxidant activity in concentration dependent manner and activity was always greater than rGO. The highest activity was shown by Neem/NiNPs where inhibition was 78% for a 250 ug/ml sample. Energy Dispersive X-Ray (EDX) analysis, shows signals of Ni along with oxygen and carbon confirming the incorporation of NiNPs into composite. Among composites, the highest activity was shown by Ni/rGO (RT) where inhibition was 63% and EC50 was 35ug/ml. This contained only 17 weight% Ni. These results confirm that after the formation of the composite, even a lower amount of NiNPs show considerably high activity due to the formation of mono-dispersed layer of NPs and phytochemicals attached on rGO. Antioxidant activity has greatly enhanced due to the synergetic effects between NiNPs and rGO sheets. These results suggest that Neem extract can be extensively used in green production of Ni/rGO composite which is an excellent candidate for safe biomedical, pharmaceutical as well as other industrial applications.
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